|Extension of multidimensional microscopy to
ultra-sensitive applications with maximum-likelihood analysisJustin Crawford, William Robinson, Guoqing Shen, Lloyd Davis
We have developed and used Monte Carlo simulation programs for studies of the capabilities of maximum-likelihood based algorithms for unmixing the contributions from different species in multi-channel spectroscopic measurements and imaging experiments. Results of some of the studies are presented here:
“Extension of multidimensional microscopy to ultrasensitive applications with maximum-likelihood analysis,” Lloyd M. Davis and Guoqing Shen, in Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, ed. by J.-A. Conchello, C.J. Cogswell, T. Wilson,Proceedings of SPIE 6443, 64430N, 1-12 (2007).
The algorithms have since been incorporated into a program written in C/C++ that can be guided by the user to either generate new simulated data or perform analysis on previously simulated, or experimental data. To download a zip file containing the C++ code, input files, and executable click here: